dh5α (New England Biolabs)
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New England Biolabs
dh5α
Dh5α, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3654 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e+coli+neb+5+alpha/NEB+5-alpha+Competent+E%2E+coli/pmc12796543-239-6-7
Average 99 stars, based on 3654 article reviews
Dh5α, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3654 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e+coli+neb+5+alpha/NEB+5-alpha+Competent+E%2E+coli/pmc12796543-239-6-7
Average 99 stars, based on 3654 article reviews
dh5α - by Bioz Stars,
2026-10
99/100 stars
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Plasmid Preparation:Article Title: Automated Cell-free Protein Synthesis for Distributed Biomanufacturing Article Snippet: E. coli BL21 (DE3) (NEB, C2527I), E. coli BL21 Star (DE3) (Invitrogen, C601003), ClearColi BL21(DE3) (Biosearch Technologies, 60810-1), and E. coli SHuffle (NEB, C3028J) strains were used to prepare cell-free lysates. .. Article Title: Multimodal diffusion for joint design of protein sequence and structure. Article Snippet: The GFP sequences were codon optimized for E. coli expression using Integrated DNA Technologies (IDT) Codon Optimization tool, and gene fragments for GFP variants were obtained from IDT (eBlocks Gene Fragments). .. The plasmid backbone and gene fragments were amplified using Phusion High-Fidelity DNA Polymerase (Thermo Scientific) with compatible overhang sequences, cloned using Gibson assembly, and transformed into Article Title: Convergent evolution of antibiotic resistance mechanisms between pyrrolobenzodiazepines and albicidin in multidrug resistant Klebsiella pneumoniae Article Snippet: Codon-optimised (DNASTAR Lasergene 16) synthetic K. oxytoca and K. pneumoniae albAS (Supplementary Table ) were cloned into pET28a(+) using Gibson assembly with the primer pairs listed (Supplementary Table ). .. The plasmids were initially transformed into Article Title: Multimodal diffusion for joint design of protein sequence and structure Article Snippet: The GFP sequences were codon optimized for E. coli expression using Integrated DNA Technologies (IDT) Codon Optimization tool, and gene fragments for GFP variants were obtained from IDT (eBlocks Gene Fragments). .. The plasmid backbone and gene fragments were amplified using Phusion High‐Fidelity DNA Polymerase (Thermo Scientific) with compatible overhang sequences, cloned using Gibson assembly, and transformed into Article Title: International Multi-site Implementation of Local Cell-Free Protein Biomanufacturing to Advance Health and Research Equity Article Snippet: .. Article Title: Bioconjugate vaccines' synthesis in prokaryotic cell lysates Article Snippet: .. Cloning:Article Title: Automated Cell-free Protein Synthesis for Distributed Biomanufacturing Article Snippet: E. coli BL21 (DE3) (NEB, C2527I), E. coli BL21 Star (DE3) (Invitrogen, C601003), ClearColi BL21(DE3) (Biosearch Technologies, 60810-1), and E. coli SHuffle (NEB, C3028J) strains were used to prepare cell-free lysates. .. Article Title: International Multi-site Implementation of Local Cell-Free Protein Biomanufacturing to Advance Health and Research Equity Article Snippet: .. Article Title: Bioconjugate vaccines' synthesis in prokaryotic cell lysates Article Snippet: .. Purification:Article Title: Automated Cell-free Protein Synthesis for Distributed Biomanufacturing Article Snippet: E. coli BL21 (DE3) (NEB, C2527I), E. coli BL21 Star (DE3) (Invitrogen, C601003), ClearColi BL21(DE3) (Biosearch Technologies, 60810-1), and E. coli SHuffle (NEB, C3028J) strains were used to prepare cell-free lysates. .. Article Title: International Multi-site Implementation of Local Cell-Free Protein Biomanufacturing to Advance Health and Research Equity Article Snippet: .. Article Title: Bioconjugate vaccines' synthesis in prokaryotic cell lysates Article Snippet: .. Amplification:Article Title: Multimodal diffusion for joint design of protein sequence and structure. Article Snippet: The GFP sequences were codon optimized for E. coli expression using Integrated DNA Technologies (IDT) Codon Optimization tool, and gene fragments for GFP variants were obtained from IDT (eBlocks Gene Fragments). .. The plasmid backbone and gene fragments were amplified using Phusion High-Fidelity DNA Polymerase (Thermo Scientific) with compatible overhang sequences, cloned using Gibson assembly, and transformed into Article Title: Multimodal diffusion for joint design of protein sequence and structure Article Snippet: The GFP sequences were codon optimized for E. coli expression using Integrated DNA Technologies (IDT) Codon Optimization tool, and gene fragments for GFP variants were obtained from IDT (eBlocks Gene Fragments). .. The plasmid backbone and gene fragments were amplified using Phusion High‐Fidelity DNA Polymerase (Thermo Scientific) with compatible overhang sequences, cloned using Gibson assembly, and transformed into Clone Assay:Article Title: Multimodal diffusion for joint design of protein sequence and structure. Article Snippet: The GFP sequences were codon optimized for E. coli expression using Integrated DNA Technologies (IDT) Codon Optimization tool, and gene fragments for GFP variants were obtained from IDT (eBlocks Gene Fragments). .. The plasmid backbone and gene fragments were amplified using Phusion High-Fidelity DNA Polymerase (Thermo Scientific) with compatible overhang sequences, cloned using Gibson assembly, and transformed into Article Title: Multimodal diffusion for joint design of protein sequence and structure Article Snippet: The GFP sequences were codon optimized for E. coli expression using Integrated DNA Technologies (IDT) Codon Optimization tool, and gene fragments for GFP variants were obtained from IDT (eBlocks Gene Fragments). .. The plasmid backbone and gene fragments were amplified using Phusion High‐Fidelity DNA Polymerase (Thermo Scientific) with compatible overhang sequences, cloned using Gibson assembly, and transformed into Transformation Assay:Article Title: Multimodal diffusion for joint design of protein sequence and structure. Article Snippet: The GFP sequences were codon optimized for E. coli expression using Integrated DNA Technologies (IDT) Codon Optimization tool, and gene fragments for GFP variants were obtained from IDT (eBlocks Gene Fragments). .. The plasmid backbone and gene fragments were amplified using Phusion High-Fidelity DNA Polymerase (Thermo Scientific) with compatible overhang sequences, cloned using Gibson assembly, and transformed into Article Title: Convergent evolution of antibiotic resistance mechanisms between pyrrolobenzodiazepines and albicidin in multidrug resistant Klebsiella pneumoniae Article Snippet: Codon-optimised (DNASTAR Lasergene 16) synthetic K. oxytoca and K. pneumoniae albAS (Supplementary Table ) were cloned into pET28a(+) using Gibson assembly with the primer pairs listed (Supplementary Table ). .. The plasmids were initially transformed into Article Title: Multimodal diffusion for joint design of protein sequence and structure Article Snippet: The GFP sequences were codon optimized for E. coli expression using Integrated DNA Technologies (IDT) Codon Optimization tool, and gene fragments for GFP variants were obtained from IDT (eBlocks Gene Fragments). .. The plasmid backbone and gene fragments were amplified using Phusion High‐Fidelity DNA Polymerase (Thermo Scientific) with compatible overhang sequences, cloned using Gibson assembly, and transformed into Article Title: Engineering auxin degradation into root-associated bacteria promotes plant growth Article Snippet: PCR products were purified using the DNA Clean & Concentrator Kit (Zymo Research) and assembled into pMo130 using HiFi Gibson Assembly Master Mix (New England Biolabs). .. Assembled plasmids were transformed into Isolation:Article Title: Convergent evolution of antibiotic resistance mechanisms between pyrrolobenzodiazepines and albicidin in multidrug resistant Klebsiella pneumoniae Article Snippet: Codon-optimised (DNASTAR Lasergene 16) synthetic K. oxytoca and K. pneumoniae albAS (Supplementary Table ) were cloned into pET28a(+) using Gibson assembly with the primer pairs listed (Supplementary Table ). .. The plasmids were initially transformed into Sequencing:Article Title: Convergent evolution of antibiotic resistance mechanisms between pyrrolobenzodiazepines and albicidin in multidrug resistant Klebsiella pneumoniae Article Snippet: Codon-optimised (DNASTAR Lasergene 16) synthetic K. oxytoca and K. pneumoniae albAS (Supplementary Table ) were cloned into pET28a(+) using Gibson assembly with the primer pairs listed (Supplementary Table ). .. The plasmids were initially transformed into Article Title: Engineering auxin degradation into root-associated bacteria promotes plant growth Article Snippet: PCR products were purified using the DNA Clean & Concentrator Kit (Zymo Research) and assembled into pMo130 using HiFi Gibson Assembly Master Mix (New England Biolabs). .. Assembled plasmids were transformed into DNA Sequencing:Article Title: Convergent evolution of antibiotic resistance mechanisms between pyrrolobenzodiazepines and albicidin in multidrug resistant Klebsiella pneumoniae Article Snippet: Codon-optimised (DNASTAR Lasergene 16) synthetic K. oxytoca and K. pneumoniae albAS (Supplementary Table ) were cloned into pET28a(+) using Gibson assembly with the primer pairs listed (Supplementary Table ). .. The plasmids were initially transformed into other:Article Title: Protocol for conditional gene expression in Magnaporthe oryzae via fungal nitrate reductase promoter replacement Article Snippet: |
